Cmb Plasmids

Lecture 2 Bacterial Plasmids What argon plasmids, and why are they so useful? [pic] What is a plasmid? |  |A plasmid is an extra-chromosomal element, a great deal a circular DNA. The plasmids we testament use in this break| | |typically fall in lead important elements: | | |A re-create site (a place to hold in foreign DNAs) | | |An origin of replica | | |A selectable marker divisor (e.g. resistance to ampicillin) | |Cloning sites |A cloning site is not required at all, exactly it sure is nice to have! What I mean by cloning site is a | | |place where the DNA rat be digested by specialised restriction enzymes - a demonstrate of entry or psychoanalysis for | | |genetic engineering work. This is a matter we will be discussing in great expatiate at a afterward point.

For | | |now, think of the following sheath: Suppose you are truly thirsty and you buy a grass of beer. Does it | | |occur to you that unitary end of the can (the round top) is d! esigned so that you can circularize it easily? If you | | |bought a can of beer with cardinal bottom ends and no top, you would have a hard duration absorbing it! Its the | | |same way with plasmids. You can have a plasmid with lots of terrific features, merely you might overleap an | | |easy way of stoolting it open with restriction enzymes. All this pour forth is making me thirsty, and now I | | |believe Ill have that drink! | |Origins of replication:...If you want to get a full essay, gild it on our website: BestEssayCheap.com

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